On the hydrodynamic analysis of macromolecular conformation
Identifieur interne : 004075 ( Main/Exploration ); précédent : 004074; suivant : 004076On the hydrodynamic analysis of macromolecular conformation
Auteurs : Stephen E. Harding [Royaume-Uni]Source :
- Biophysical Chemistry [ 0301-4622 ] ; 1995.
English descriptors
- Teeft :
- Ablate ellipsoid, Absolute temperature, Analytical ultracentrifugation, Analytical ultracentrifuge, Anhydrous macromolecule, Anhydrous volume, Anisotropy, Axial, Axial dimensions, Axial ratio, Axial ratios, Bead, Bead modelling, Bead models, Best model, Biochem, Biochemistry, Biol, Biomolecular, Biomolecular assemblies, Biophys, Biophysical chemistry, Biopolymers, Bloomfield, Chem, Clarendon press, Coefficient, Common intercept, Compact sphere, Comprehensive description, Conditions water, Conformation, Constant values, Continuous flexibility, Contour length, Convergence problems, Corresponding coefficient, Covolume, Crystal coordinates, Decay constants, Dichroism, Double logarithmic plot, Dynamic light, Dynamic properties, Electric birefringence, Electric birefringence decay, Electric birefringence decay constants, Electric dichroism, Electron microscopy, Ellipsoid, Ellipsoid modelling, Elliptic, Elliptic integrals, Elliptic type, Experimental error, Experimental parameters, Finite concentration, Flexible joints, Fluid mech, Frictional, Frictional coefficient, Frictional properties, Frictional ratio, Fuller description, Garcia, Garcia bernal, General conformation, General ellipsoid, General ellipsoids, General triaxial ellipsoid, General triaxial ellipsoids, Globular particles, Glycoprotein, Good agreement, Graphical intersection, Gross conformation, Gyration, Harding, Haug triangle, High sensitivity, Horton, Hydration, Hydrodynamic, Hydrodynamic parameters, Hydrodynamic properties, Increment, Inherent viscosity, Intrinsic viscosities, Intrinsic viscosity, Ionic strength, Laser, Laser light, Line solutions, Lopez martinez, Macromolecular, Macromolecular conformation, Macromolecular system, Macromolecule, Many classes, Many polysaccharides, Mhks, Mhks coefficients, Modelling, Modelling strategies, Molar, Molar covolume, Molar mass, Molecular weight, Molecular weights, Monodisperse solution, Mucus glycoproteins, Myosin, Myosin example, Neurophysin, Neurophysin monomers, Nucleic acids, Oblate, Oblate ellipsoids, Other combinations, Parameter, Particle conformation, Pectin, Permeation chromatography, Persistence length, Phys, Physical chemistry, Point mutants, Polydisperse systems, Polymer, Polymer chain, Polymer science, Polysaccharide, Poor sensitivity, Prolate, Prolate ellipsoid, Prolate ellipsoids, Random coil, Random coils, Ratio function, Relaxation times, Revolution modelling, Revolution shape parameters, Rigid spheres, Rotational, Rotational diffusion, Rotational diffusion coefficients, Rotational relaxation time, Rotational relaxation times, Rowe, Royal society, Same mass, Sedimentation, Sedimentation coefficient, Sedimentation coefficients, Sedimentation equilibrium, Sedimentation velocity, Segmental flexibility, Semi axes, Sensitive function, Shape functions, Shape information, Shape parameters, Similar approach, Source functions, Source references, Special publication, Specific volume, Spherical particle, Standard conditions, Surface water, Torre, Translational, Translational diffusion coefficient, Triaxial, Triaxial ellipsoid, Unit mass, Virial coefficient, Viscosity increment.
Abstract
Abstract: Hydrodynamics provides a powerful complementary role to the traditional “high resolution” techniques for the investigation of macromolecular conformation, especially in dilute solution, conditions which are generally inaccessible to other structural techniques. This paper describes the state of art of hydrodynamic representations for macromolecular conformation, in terms of (1) simple but straightforward ellipsoid of revolution modelling; (2) general triaxial ellipsoid modelling; (3) hydrodynamic bead modelling; (4) the ability, especially for polydisperse macromolecular systems, to distinguish between various conformation types; (5) analysis of macromolecular flexibility.
Url:
DOI: 10.1016/0301-4622(94)00143-8
Affiliations:
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Le document en format XML
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<term>Absolute temperature</term>
<term>Analytical ultracentrifugation</term>
<term>Analytical ultracentrifuge</term>
<term>Anhydrous macromolecule</term>
<term>Anhydrous volume</term>
<term>Anisotropy</term>
<term>Axial</term>
<term>Axial dimensions</term>
<term>Axial ratio</term>
<term>Axial ratios</term>
<term>Bead</term>
<term>Bead modelling</term>
<term>Bead models</term>
<term>Best model</term>
<term>Biochem</term>
<term>Biochemistry</term>
<term>Biol</term>
<term>Biomolecular</term>
<term>Biomolecular assemblies</term>
<term>Biophys</term>
<term>Biophysical chemistry</term>
<term>Biopolymers</term>
<term>Bloomfield</term>
<term>Chem</term>
<term>Clarendon press</term>
<term>Coefficient</term>
<term>Common intercept</term>
<term>Compact sphere</term>
<term>Comprehensive description</term>
<term>Conditions water</term>
<term>Conformation</term>
<term>Constant values</term>
<term>Continuous flexibility</term>
<term>Contour length</term>
<term>Convergence problems</term>
<term>Corresponding coefficient</term>
<term>Covolume</term>
<term>Crystal coordinates</term>
<term>Decay constants</term>
<term>Dichroism</term>
<term>Double logarithmic plot</term>
<term>Dynamic light</term>
<term>Dynamic properties</term>
<term>Electric birefringence</term>
<term>Electric birefringence decay</term>
<term>Electric birefringence decay constants</term>
<term>Electric dichroism</term>
<term>Electron microscopy</term>
<term>Ellipsoid</term>
<term>Ellipsoid modelling</term>
<term>Elliptic</term>
<term>Elliptic integrals</term>
<term>Elliptic type</term>
<term>Experimental error</term>
<term>Experimental parameters</term>
<term>Finite concentration</term>
<term>Flexible joints</term>
<term>Fluid mech</term>
<term>Frictional</term>
<term>Frictional coefficient</term>
<term>Frictional properties</term>
<term>Frictional ratio</term>
<term>Fuller description</term>
<term>Garcia</term>
<term>Garcia bernal</term>
<term>General conformation</term>
<term>General ellipsoid</term>
<term>General ellipsoids</term>
<term>General triaxial ellipsoid</term>
<term>General triaxial ellipsoids</term>
<term>Globular particles</term>
<term>Glycoprotein</term>
<term>Good agreement</term>
<term>Graphical intersection</term>
<term>Gross conformation</term>
<term>Gyration</term>
<term>Harding</term>
<term>Haug triangle</term>
<term>High sensitivity</term>
<term>Horton</term>
<term>Hydration</term>
<term>Hydrodynamic</term>
<term>Hydrodynamic parameters</term>
<term>Hydrodynamic properties</term>
<term>Increment</term>
<term>Inherent viscosity</term>
<term>Intrinsic viscosities</term>
<term>Intrinsic viscosity</term>
<term>Ionic strength</term>
<term>Laser</term>
<term>Laser light</term>
<term>Line solutions</term>
<term>Lopez martinez</term>
<term>Macromolecular</term>
<term>Macromolecular conformation</term>
<term>Macromolecular system</term>
<term>Macromolecule</term>
<term>Many classes</term>
<term>Many polysaccharides</term>
<term>Mhks</term>
<term>Mhks coefficients</term>
<term>Modelling</term>
<term>Modelling strategies</term>
<term>Molar</term>
<term>Molar covolume</term>
<term>Molar mass</term>
<term>Molecular weight</term>
<term>Molecular weights</term>
<term>Monodisperse solution</term>
<term>Mucus glycoproteins</term>
<term>Myosin</term>
<term>Myosin example</term>
<term>Neurophysin</term>
<term>Neurophysin monomers</term>
<term>Nucleic acids</term>
<term>Oblate</term>
<term>Oblate ellipsoids</term>
<term>Other combinations</term>
<term>Parameter</term>
<term>Particle conformation</term>
<term>Pectin</term>
<term>Permeation chromatography</term>
<term>Persistence length</term>
<term>Phys</term>
<term>Physical chemistry</term>
<term>Point mutants</term>
<term>Polydisperse systems</term>
<term>Polymer</term>
<term>Polymer chain</term>
<term>Polymer science</term>
<term>Polysaccharide</term>
<term>Poor sensitivity</term>
<term>Prolate</term>
<term>Prolate ellipsoid</term>
<term>Prolate ellipsoids</term>
<term>Random coil</term>
<term>Random coils</term>
<term>Ratio function</term>
<term>Relaxation times</term>
<term>Revolution modelling</term>
<term>Revolution shape parameters</term>
<term>Rigid spheres</term>
<term>Rotational</term>
<term>Rotational diffusion</term>
<term>Rotational diffusion coefficients</term>
<term>Rotational relaxation time</term>
<term>Rotational relaxation times</term>
<term>Rowe</term>
<term>Royal society</term>
<term>Same mass</term>
<term>Sedimentation</term>
<term>Sedimentation coefficient</term>
<term>Sedimentation coefficients</term>
<term>Sedimentation equilibrium</term>
<term>Sedimentation velocity</term>
<term>Segmental flexibility</term>
<term>Semi axes</term>
<term>Sensitive function</term>
<term>Shape functions</term>
<term>Shape information</term>
<term>Shape parameters</term>
<term>Similar approach</term>
<term>Source functions</term>
<term>Source references</term>
<term>Special publication</term>
<term>Specific volume</term>
<term>Spherical particle</term>
<term>Standard conditions</term>
<term>Surface water</term>
<term>Torre</term>
<term>Translational</term>
<term>Translational diffusion coefficient</term>
<term>Triaxial</term>
<term>Triaxial ellipsoid</term>
<term>Unit mass</term>
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<front><div type="abstract" xml:lang="en">Abstract: Hydrodynamics provides a powerful complementary role to the traditional “high resolution” techniques for the investigation of macromolecular conformation, especially in dilute solution, conditions which are generally inaccessible to other structural techniques. This paper describes the state of art of hydrodynamic representations for macromolecular conformation, in terms of (1) simple but straightforward ellipsoid of revolution modelling; (2) general triaxial ellipsoid modelling; (3) hydrodynamic bead modelling; (4) the ability, especially for polydisperse macromolecular systems, to distinguish between various conformation types; (5) analysis of macromolecular flexibility.</div>
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